HT280T Headspace, Liquid and SPME Autosampler

The HT280T is a single unit combining static headspace analysis, liquid sample injection and SPME (Solid Phase Microextraction)!

As the successor to the hugely popular HT250D, the HT280T effectively combines the operations of the HT200H Headspace Autosampler and the HT300A Liquid Autosampler, with the addition of SPME.

Consider that the HT280T is compatible with almost all GC and GC/MS systems, both present and past, and you have a truly remarkable development in GC autosampling. The HT280T can save you time and money, increase analysis flexibility and save older GCs from redundancy.

Changing between liquid, headspace and SPME modes is very simple. It takes about 5 minutes and there's no need to remove the sampler from the GC.

Headspace Operation

The system is uses a heated syringe to extract headspace samples from the 6 position orbital oven/shaker. This eliminates tubing, dead volume and sample absorption.

Vial transport is positive and incredibly reliable — the system constantly checks that vials are present and are located in the correct place. No expensive magnetic caps are required.

No transfer lines are required and the unit mounts directly on the GC thus reducing bench space requirements. Operation is via the simple keypad or by HT-COMSoft software. Up to 40 headspace vials of 20ml or 10ml may be stored in the standard tray.

Progressive sample preparation means that samples are automatically loaded into the oven at the correct time to ensure an injection is ready as soon as the previous run is complete, maximizing your GC efficiency.

Key Features:
• No transfer lines means no sample crossover
• Rotating head design leaves injection port free for manual injection
• Progressive sample transfer means samples always ready when previous run completed
• May be configured for left or right operation
• Gas flush of syringe between injections

Liquid Sampler Operation

Up to 110 samples may be processed as single or batch injections using one or more analysis methods. Any 12x32mm vial may be used with any cap type. All parameters from the sampling depth through to the injection speed, depth and dwell times may be programmed through the front panel or via the HT-COMSoft software

The automatic injection sequence may have up to 15 steps which can be programmed to include:
• First and last samples of group
• Injection method
• Number of injections for each sample
• Pre- and Post-washing solvent position
• Internal Standard (if used)

Key Features:
• No transfer lines means no sample crossover
• Syringe may be washed with solvent or sample
• Injections may be made on two columns (high throughput and confirmation mode)
• Rotating head design leaves injection port free for manual injection
• Internal standard sampling
• Memory stores 10 different methods
• Variable fill speed allows for wide range of sample viscosities
• Sampling system eliminates air bubbles
• Programmable sampling and injection speed

SPME: When placed into the headspace gas or a liquid, the compounds adsorb onto the fibre

SPME Operation

SPME is a unique sample preparation technique that eliminates most of the drawbacks associated with extracting organics. SPME requires no solvents or complicated apparatus.

SPME has gained widespread acceptance as the technique of preference for many applications including: flavours, fragrances and contaminants in food; forensic and toxicology applications; environmental and biological matrices; organic volatiles in pharmaceutical compounds.

Automated SPME with the HT280T delivers more accurate results with greater throughput than manual SPME. The HT280T can extract volatile and non-volatile compounds in both liquid and headspace samples using variable vial penetration depth. Samples can be derivatised pre or post-extraction as the application requires.

The extraction is performed by exposing the fibre into the sample vial. Samples can be agitated by orbital rotation and heated during extraction. Both the shaking speed and oven temperature are programmable. The oven door is kept closed during extraction to maintain constant temperature.

After the compounds have been thermally desorbed in the GC injector, the fibre may be fully cleaned again in the optional heated fibre cleaning station positioned at the back of the unit.

Key Features:
• Single step extraction (reduces sample preparation time by up to 70%)
• Minimal use of solvents
• Programmable extraction depth to perform both headspace and liquid extraction
• Oven door kept closed during extraction to keep temperature constant

Specifications
GC Compatibility:	Agilent/HP 4890 Agilent/HP 5890 Agilent/HP 6850A Agilent/HP 6890 Agilent/HP 7890A Carlo Erba 5300 Mega Carlo Erba 8000 Carlo Erba 8000 Top Carlo Erba 8500 Mega 2 Chemito 1000 Chrompack CP9001/2 Dani 8610/8500 Dani Master GC Donam DS6200 DPS Instruments Fuli Instruments Gow Mac* Kristallux 4000M Meta Chrom* Perichrom Perkin-Elmer Clarus 400 / Clarus 600 Perkin Elmer XL Shimadzu GC-14A/B/C Shimadzu GC-17A Shimadzu GC-2010 Shimadzu GC-2014 Shimadzu QP-2010 SRI 8610* Techcomp Trace SX Varian 430/450 Varian 3300/3400 Varian 3800 Varian 3900 Younglin* * Only if specified when ordering the GC If your GC is not listed it may still be compatible. Please contact us for details.
Data System Compatibility:	EZChrom Elite and DataApex Clarity (Headspace and liquid modes only)
HEADSPACE OPERATION
Shaking Method:	Orbital
Incubation Oven:	6 position
Syringe Size:	2.5ml
Tray Capacity:	40 Vials, 10 or 20ml
Cleaning System:	Nitrogen flush
Conditioning
Oven Temperature:	40 - 150°C
Time:	0 - 23h 59m
Progressive Increase:	0 - 9h 59m
Shaker Speed:	320 - 720rpm
Shaking Cycles On/Off:	0 - 9.9mins
Sampling
Syringe Temperature:	40 - 150°C
Pre-fill Volume:	Steps of 0.01ml
Pull Up Strokes:	Up to 15 Strokes
Equilibrium Delay:	Up to 60secs
Sampling Volume:	Steps of 0.01ml
Filling Speed:	0.1 - 100ml/min
Injection
Sampling Repeats:	Up to 15
Waiting Time between Samples:	0 - 99mins
Injection Speed:	0.1 - 100ml/min
Waiting Time (before and after injection):	0 - 99secs
LIQUID OPERATION
Syringe Sizes:	1, 10, 25, 50 & 100µl
Tray Capacity:	110 Vials, 2 or 2.5ml
Sample Volume:	Steps of 0.1µl
Air Volume:	Steps of 0.1µl
Aspirating Speed:	1 - 100µl/sec
Needle Washing:	Up to 15 Strokes
Washing Mode:	Every injection, sample or step
Air Bubble Removing:	Up to 15 Strokes
Viscosity Time:	0 - 15secs
Injection
Injection Speed:	0.1 - 100µl/sec
Waiting Time (before and after injection):	0 - 99secs
Injection Depth:	Variable
Internal Standard Technique
IS Volume:	Steps of 0.1µl
Air Gap Volume:	Steps of 0.1µl
Mode:	1 or 2 air gaps
SPME OPERATION
Extraction:	Liquid and Headspace
Tray Capacity:	40 Vials, 10 or 20ml
Extraction Depth:	Variable
Shaking Method:	Orbital
Incubation Oven:	6 position
Oven Temperature:	40 - 150°C
Shaker Speed:	320 - 720rpm
Oven Door:	Kept closed during extraction
Fibre Cleaning Station (optional):	Variable duration
GENERAL
Control
Electrical Control:	RS232 and TTL
Physical Characteristics
Dimensions (W x H x D):	420 x 620 x 400mm
Mass:	11.5kg

Additional Information

HT280T Datasheet (475KB)

Pricing

Description	Part No	Qty	List Price
HT280T Headspace, Liquid and SPME Autosampler	HT280T	Each